B12

Vitamin B12 (specifically Methylcobalamin) is a critical micronutrient that acts as a cofactor for DNA synthesis, fatty acid metabolism, and amino acid metabolism. In research and clinical settings, injectable B12 is preferred over oral supplements because it bypasses the digestive system, ensuring 100% bioavailability.
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1. Types of B12
There are two primary forms of injectable B12 used in research:
• Methylcobalamin: The "active" coenzyme form. It is more easily utilized by the central nervous system and is the gold standard for brain health and focus.
• Cyanocobalamin: A synthetic form that the body must convert into Methylcobalamin. It is cheaper but less efficient for neurological support.
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2. Dosage & Concentration
B12 is usually provided as a pre-mixed liquid vial. Common concentrations are 1,000mcg/ml or 5,000mcg/ml.

• Standard Research Dose: 1,000mcg (1mg) per injection.
• High-Intensity Dose: 2,000mcg to 5,000mcg (often used for severe deficiency or high-level athletic recovery).

• Syringe Measurement:
o If the vial is 1,000mcg/ml, a 1ml (100 units) dose = 1,000mcg.
o If the vial is 5,000mcg/ml, a 0.2ml (20 units) dose = 1,000mcg.
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3. The Protocol & Timing
Because B12 is water-soluble, the body flushes out what it cannot use. Therefore, frequency is more important than a single massive dose.

Goal Frequency Duration
Maintenance 1x Weekly Indefinite
Energy/Recovery 2-3x Weekly 8-12 Weeks
Loading Phase Daily (for 7 days) 1 Week (then shift to weekly)

Timing: Always administer in the morning. B12 is involved in ATP (energy) production; taking it at night can cause insomnia or unusually vivid dreams.
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4. Administration: IM vs. SubQ

• Intramuscular (IM): The traditional clinical method. It allows for a slightly slower, more sustained release into the bloodstream. Best sites are the Deltoid or Glute.

• Subcutaneous (SubQ): Perfectly acceptable for B12. It is less painful and easier for self-administration. Inject into the stomach fat or thigh.
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5. Potential Side Effects
• The "B12 Glow": Your urine may turn a darker yellow or even have a slight pinkish hue (if using very high doses). This is normal.
• B12 Acne: In some individuals, high-dose B12 can alter the gene expression of skin bacteria, leading to temporary breakouts.
• Hypokalemia: Rare, but high-dose B12 therapy can cause potassium levels to drop as the body produces new red blood cells. Ensuring adequate potassium intake (bananas, electrolytes) is recommended.
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Quick Checklist for a 30ml Vial:
• At 1ml/week: The vial lasts 30 weeks.
• Storage: B12 is extremely light-sensitive. Keep the vial in its box or wrap it in foil, and store it in the refrigerator.
• Supplies: 25g/27g needles for IM or 31g insulin syringes for SubQ.